First biochemical characterization of a novel ribonuclease from wild mushroom Amanita hemibapha

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First biochemical characterization of a novel ribonuclease from wild mushroom Amanita hemibapha

A 45-kDa ribonuclease (RNase) was purified from dried fruiting bodies of the wild mushroom Amanita hemibapha. It was adsorbed on DEAE-cellulose, S-sepharose, and finally purified on Superdex 75. The RNase exhibited maximal RNase activity at pH 5 and in a temperature range between 60-70°C. It demonstrated no ribonucleolytic activity toward four polyhomoribonucleotides. The amino acid sequence an...

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Clinical characteristics and outcome of toxicity from Amanita mushroom poisoning

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ژورنال

عنوان ژورنال: SpringerPlus

سال: 2012

ISSN: 2193-1801

DOI: 10.1186/2193-1801-1-79