First biochemical characterization of a novel ribonuclease from wild mushroom Amanita hemibapha
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چکیده
منابع مشابه
First biochemical characterization of a novel ribonuclease from wild mushroom Amanita hemibapha
A 45-kDa ribonuclease (RNase) was purified from dried fruiting bodies of the wild mushroom Amanita hemibapha. It was adsorbed on DEAE-cellulose, S-sepharose, and finally purified on Superdex 75. The RNase exhibited maximal RNase activity at pH 5 and in a temperature range between 60-70°C. It demonstrated no ribonucleolytic activity toward four polyhomoribonucleotides. The amino acid sequence an...
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A 15 kDa ribonuclease (RNase) was purified from dried fruiting bodies of the wild edible mushroom Armillaria luteo-virens. The simple 4-step purification protocol involved ion-exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion-exchange chromatography on SP-Sepharose and a final gel filtration by FPLC on Superdex-75. The RNase was unadsorbed on Affi-gel ...
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ژورنال
عنوان ژورنال: SpringerPlus
سال: 2012
ISSN: 2193-1801
DOI: 10.1186/2193-1801-1-79